Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00035
OS Name
Light-trigggered recruitment of G protein signaling protein system (LTR-RGS)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Recruited protein Cryptochrome-interacting basic-helix-loop-helix 1
Detail Info
Fusion protein Regulator of G-protein signaling 4
Detail Info
OS Application Information (1)
Mechanism of Action CRY2PHR-CIBN heterodimerization [ 1]
Controlled Signal Pathway Inhibit G protein signal
Effect Inhibition
Application Inhibit G protein generate signal and cell migration
Note Locally inhibit the activity of specific G protein subunits
Light Information Activation Wavelength 445 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Minutes
Deactivation Time Minutes
Light Intensity 5 microwatts
Expression Information Expression Method Transfection
Cell Lines Macrophage cells line RAW264.7 and Hela cells
References
1 Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration