Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00034
OS Name
Light induced PIP3 production system (PI3K)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Recruited protein Cryptochrome-interacting basic-helix-loop-helix 1
Detail Info
Fusion protein Inter-SH2 domain of p85beta
Detail Info
OS Application Information (1)
Mechanism of Action CRY2PHR-CIBN heterodimerization [ 1]
Controlled Signal Pathway PIP3 production
Effect Activation
Application Induce PIP3 production at sepecific location to munipulate cell activity via endocytosis
Light Information Activation Wavelength 442 nm or 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Minutes
Expression Information Expression Method Transfection
Cell Lines Hippocampal neurons and HEK293 cells
References
1 Optogenetic control of PIP3: PIP3 is sufficient to induce the actin-based active part of growth cones and is regulated via endocytosis