Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00032
|
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| OS Name |
DCas9-based optical gene activation system
(Opto-dCas9)
|
[1], [2] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Recruited protein | CRISPR-associated endonuclease Cas9 | ||||
| Fusion protein | Transcription factor p65 | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2-CIB1 heterodimerization | [ 2] | ||||
| Controlled Signal Pathway | Regulate mRNA transcription | |||||
| Effect | Activation | |||||
| Application | Light activated CRISPR activation system to control gene expression | |||||
| Light Information | Activation Wavelength | 390-480 nm | ||||
| Activation Time | hours | |||||
| Light Intensity | 1 watts per square meter | |||||
| Expression Information | Expression Method | Nucleofection and Transfection | ||||
| Cell Lines | Zebrafish embryonic fibroblast cells | |||||
| OS Application Information (2) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2PHRCCIBN heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Gene expression activation | |||||
| Effect | Activation | |||||
| Application | CRISPR-CAS9 based photoactivable transcription | |||||
| Note | Based on dCAS9; synergistically enhanced by multiple sgRNAs | |||||
| Light Information | Activation Wavelength | 470 ± 20 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | Protein interact within second while take hours to detect mRNA | |||||
| Deactivation Time | Hours | |||||
| Light Intensity | 1.5 watts per square meter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | HEK293 cells; Hela cells; COS-7 cells and HEK293T cells | |||||