Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID	OS00030
OS Name	genetically encoded light-inducible protein-interaction system (LIPI)		[1]
Photoreceptor (PR) PR Info	PR Name Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR) Source of Species Arabidopsis thalino Class Cryptochromes UniProt ID Q96524 GenBank NP_849588 PDB ID 6X24 3D Structure Components Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR) Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein	Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor	Flavin adenine dinucleotide (FAD)
Controlled Protein	Functional protein	Cryptochrome-2	Detail Info
Controlled Protein	Recruited protein	Cryptochrome-interacting basic-helix-loop-helix 1	Detail Info

OS Application Information (1)
Mechanism of Action	CRY2-CIB1 heterodimerization		[ 1]
Controlled Signal Pathway	Protein translocation
Effect	Activation
Application	Cre reconmbinase mediated DNA recombination to regulate gene expression
Note	Dim room light wont triger translocation
Light Information	Activation Wavelength	488 nm
	Process	Reversible
	Deactivation Wavelength	Dark
	Activation Time	300 ms
	Deactivation Time	5.5 min
	Light Intensity	25 microwatts
Expression Information	Expression Method	Transfection
Expression Information	Cell Lines	HEK293T cells

References

Rapid blue-light-mediated induction of protein interactions in living cells