Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00029
OS Name
Light inducible nuclear translocation and dimerization system (LINTAD)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein LexA activation domain
Detail Info
Recruited protein Transcription regulator LexA
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIB1 heterodimerization [ 1]
Controlled Signal Pathway Gene expression activation
Effect Activation
Application Control transcription in CAR T cell for cancer therapy
Note LexA-VP is an orthogonal transcription factor
Light Information Activation Wavelength 460 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Minutes
Light Intensity 5 milliwatts per square centimeter
Expression Information Expression Method Transduction and Transfection
Cell Lines CAR T cells
References
1 Engineering light-controllable CAR T cells for cancer immunotherapy