Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00028
OS Name
Optogenetic controlled C-RAF signal system (Opto-C-RAF)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Recruited protein Protein kinase CRAF
Detail Info
Fusion protein RAF proto-oncogene serine/threonine-protein kinase
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIBN heterodimerization and CRY2 homo-oligomerization [ 1]
Controlled Signal Pathway RAF activation
Effect Activation
Application Reversible control of protein kinase activity in signal duration and strength
Light Information Activation Wavelength 460 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Minutes
Expression Information Expression Method Transfection
Cell Lines HEK293T cells
References
1 Optogenetic control of protein kinase activity in mammalian cells