Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00026
OS Name
Optogenetic controlled Raf1 system (OptoRaf1)
 [1], [2]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Recruited protein Cryptochrome-interacting basic-helix-loop-helix 1
Detail Info
Fusion protein RAF proto-oncogene serine/threonine-protein kinase
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIBN heterodimerization [ 1] , [ 2]
Controlled Signal Pathway MAPK activation
Effect Activation
Application Control kinase activity during differentiation and embryonic development
Note 2A is a peptides function through a ribosomal skipping mechanism
Light Information Activation Wavelength 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 15 min
Deactivation Time 15 min
Light Intensity 5 milliwatts per square centimeter
Expression Information Expression Method Transfection and Transduction
Cell Lines PC12 cells and Xenopus embryos
References
1 Reversible optogenetic control of kinase activity during differentiation and embryonic development
2 Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development