Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00025
OS Name
Optogenetic controlled Wnt signaling system (OptoWnt)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Armadillo segment polarity protein
Detail Info
OS Application Information (1)
Mechanism of Action CRY2 homo-oligomerization [ 1]
Controlled Signal Pathway Wnt signaling regulation
Effect Inhibition
Application A non-invasive and versatile tool to study Wnt signaling during embryogenesis
Note Light-sheet microscopy allows observation of the full course of Drosophila embryonic development from egg to larva
Light Information Activation Wavelength 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Minutes
Deactivation Time Minutes
Light Intensity 30 milliwatts
Expression Information Expression Method Transfection
Cell Lines HEK293-Wnt3a-TOPFlash cells
References
1 Coupling optogenetics and light-sheet microscopy, a method to study Wnt signaling during embryogenesis