Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID	OS00024
OS Name	Optogenetic controlled AKT1 signaling system (OptoAKT1)		[1], [2]
Photoreceptor (PR) PR Info	PR Name Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR) Source of Species Arabidopsis thalino Class Cryptochromes UniProt ID Q96524 GenBank NP_849588 PDB ID 6X24 3D Structure Components Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR) Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein	Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor	Flavin adenine dinucleotide (FAD)
Controlled Protein	Recruited protein	Myristoylation/palmitoylation signal HCK	Detail Info
Controlled Protein	Fusion protein	RAC-alpha serine/threonine-protein kinase	Detail Info

OS Application Information (1)
Mechanism of Action	CRY2-CIBN heterodimerization		[ 1] , [ 2]
Controlled Signal Pathway	AKT activation
Effect	Sensor
Application	Precise control AKT1 mediated cell signals by light
Note	Cotransfected with CRY2-EGFP-AKT1 and m/p-mCherry-CIBN ratio 5:1
Light Information	Activation Wavelength	450 nm
	Process	Reversible
	Deactivation Wavelength	Dark
	Activation Time	15 min
	Deactivation Time	>15 min
	Light Intensity	0.5-2.6 watts per square meter
Expression Information	Expression Method	Transfection
Expression Information	Cell Lines	HEK293 cells

References

1	Multichromatic Control of Signaling Pathways in Mammalian Cells
2	Noncanonical role of Arabidopsis COP1/SPA complex in repressing BIN2-mediated PIF3 phosphorylation and degradation in darkness