Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00023
|
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| OS Name |
Optogenetic controlled Akt signal system
(Opto-Akt)
|
[1] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Recruited protein | Membrane-targeting myristoylation sequence | ||||
| Fusion protein | RAC-alpha serine/threonine-protein kinase | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2-CIBN heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | AKT activation | |||||
| Effect | Sensor | |||||
| Application | Specific regulation of Akt activity in a spatiotemporal manner within its physiological dynamic range | |||||
| Note | AKT is crucial for the regulation of diverse cellular function | |||||
| Light Information | Activation Wavelength | 440 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 5 s | |||||
| Deactivation Time | 2 min | |||||
| Light Intensity | 1 milliwatts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | C2C12 cells; HEK293 cells; and PlatE cells | |||||