Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00022
OS Name
PIP3 production by photo-activated PI3K (PPAP1.0)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Recruited protein Cryptochrome-interacting basic-helix-loop-helix 1
Detail Info
Fusion protein Phosphoinositide 3-kinase regulatory subunit 6
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIBN heterodimerization [ 1]
Controlled Signal Pathway P110 activation to transform phosphatidylinositol 4,5-bisphophate (PIP2) to PIP3
Effect Sensor
Application Light-control PIP3 production at local regions in living cells
Note Blue light irradiated PPAP1.0 generated 2.1 times more PIP3 than PPAP1.0 in the dark
Light Information Activation Wavelength 470 ± 20 nm
Activation Time 10 min
Light Intensity 0.5 watts per square meter
Expression Information Expression Method Transfection
Cell Lines HEK293 cells; NIH3T3 cells and COS7 cells
References
1 Membrane Dynamics Induced by a Phosphatidylinositol 3,4,5-Trisphosphate Optogenetic Tool