Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00021
OS Name
Optogenetic controlled iTrkB signaling system3 (Opto-iTrkB3)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Recruited protein Cryptochrome-interacting basic-helix-loop-helix 1
Detail Info
Fusion protein Intracellular Tropomyosin receptor kinase B
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIBN heterodimerization [ 1]
Controlled Signal Pathway TrkB signal activation
Effect Sensor
Application Investigate brain-drived neurotrophic factor TrkB signaling with tight spatial and temporal control
Note Most efficiently activate TrkB signaling
Light Information Activation Wavelength 470 nm
Activation Time Seconds
Light Intensity 9.7 watts per square centimeter
Expression Information Expression Method Transfection
Cell Lines PC12 cells and NIH 3T3 cells
References
1 Optical Activation of TrkB Signaling