Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00016
OS Name
Optogenetic controlled Dab1 signal system (Opto-Dab1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein DAB adaptor protein 1
Detail Info
OS Application Information (1)
Mechanism of Action CRY2 homo-oligomerization [ 1]
Controlled Signal Pathway Dab1 phosphorylation
Effect Activation
Application Study the biological function of the Reelin-Dab1 signaling pathway
Note Reelin-Dab1 signaling pathway regulate the development of the mammalian brain
Light Information Activation Wavelength 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 2 min
Deactivation Time 30 min
Expression Information Expression Method Transfection and Nucleofection
Cell Lines COS7 cells; HEK293 cells; NIH3T3 cells and primary neurons
References
1 Optogenetic control of the Dab1 signaling pathway