Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00015
OS Name
Optogenetic regulate DNA methylation system (Opto-DNAM)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Human DNA methyltransferase 3A
Detail Info
Recruited protein Telomere repeat binding factor-1
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIBN heterodimerization [ 1]
Controlled Signal Pathway Gene expression inhibition
Effect Inhibition
Application Regulate the epigenetic pathway at telomeres and other selected genomic regions of interest
Light Information Activation Wavelength 465 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 5 min
Deactivation Time 20 h
Light Intensity 8 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines Hela cells
References
1 Optogenetic regulation of site-specific subtelomeric DNA methylation