Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00014
OS Name
Photoactivated split lexA transcriptional activation system (PA-LexA)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Transcription factor p65
Detail Info
Virus protein 16
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIBN heterodimerization [ 1]
Controlled Signal Pathway Gene expression activation
Effect Activation
Application Light-inducible transcription system in Drosophila
Note Succeed in control GCaMP3.0 expression in fruitless neuron and control of courtship behavior
Light Information Activation Wavelength 474 nm
Activation Time 1 h
Light Intensity 2.5 milliwatts per square centimeter
Expression Information Expression Method Transgene
Cell Lines Drosophila
References
1 Optogenetic Control of Gene Expression in Drosophila