Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00013
OS Name
Light activated reversible inhibition by assembled trap (LARIAT)
 [1], [2], [3]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Recruited protein Multimeric protein
Detail Info
Fusion protein Vav guanine nucleotide exchange factor 2
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIB1 heterodimerization [ 1] , [ 2]
Controlled Signal Pathway Vav2 inactivation
Effect Inhibition
Application Optical inhibition of Vav2 to regulate cell migration
Light Information Activation Wavelength 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time <1 min
Deactivation Time Minutes
Light Intensity 65 microwatts per square millimeter
Expression Information Expression Method Transfection
Cell Lines Hela cells
OS Application Information (2)
Mechanism of Action CRY2 homo-oligomerization and CRY2-CIB1 heterodimerization [ 3]
Controlled Signal Pathway Protein translocation
Effect Inhibition
Application Inactivate signal protein (Vav2) by clustering
Light Information Activation Wavelength 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Minutes
Light Intensity 6.5 microwatts
Expression Information Expression Method Transfection
Cell Lines NIH 3T3 cells and Hela cells
References
1 Light-Induced Protein Clustering for Optogenetic Interference and Protein Interaction Analysis in Drosophila S2 Cells
2 Protein Inactivation by Optogenetic Trapping in Living Cells
3 Reversible protein inactivation by optogenetic trapping in cells