Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00011
OS Name
Optogenetic control of fibroblast growth factor receptor (OptoFGFR1)
 [1], [2]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Fibroblast Growth Factor Receptor 1
Detail Info
OS Application Information (1)
Mechanism of Action CRY2 homo-oligomerization [ 1] , [ 2]
Controlled Signal Pathway FGFR1 activation
Effect Activation
Application Control fibroblast growth factor receptor signaling by light
Note Not test the reversibility in this paper
Light Information Activation Wavelength 488 nm
Activation Time Minutes
Light Intensity 0.5 microwatts per square millimeter
Expression Information Expression Method Transfection
Cell Lines HeLa cells and HEK293 cells
References
1 Aminoguanidine cream ameliorates skin tissue microenvironment in diabetic rats
2 Spatiotemporal control of fibroblast growth factor receptor signals by blue light