Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00010
OS Name
Optogenetic regulated G-protein signaling system (Opto-RGS2)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Human G-protein signaling 2
Detail Info
Recruited protein Regulator of G-protein signaling 2
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIBN heterodimerization [ 1]
Controlled Signal Pathway Intracellular calcium supress
Effect Inhibition
Application Termination of Galphaq signaling and downstream release of calcium from the endoplasmic reticulum
Note RGS2 terminates activated Galphaq signaling as a GTPase accelerating protein
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 19.7 ± 2.0 s
Deactivation Time 396 ± 45 s
Light Intensity 7.37 milliwatts per square miliimeter
Expression Information Expression Method Transfection
Cell Lines HEK293T cells
References
1 Optogenetic Inhibition of G(q) Protein Signaling Reduces Calcium Oscillation Stochasticity