Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00008
OS Name
Cofilin-actin interaction optocontroled regulated system (CofActor)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Cofilin; a actin-binding protein
Detail Info
Functional protein Opto-a1AR
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIB1 heterodimerization [ 1]
Controlled Signal Pathway CofilinCactin interaction control
Effect Sensor
Application Tracking of early stage events in neurondegeneration and investigating actin interactions with other proteins during cellular stress
Note Both light and energetic oxidative stress-gated control
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 10 min
Deactivation Time Minutes
Expression Information Expression Method Transfection
Cell Lines Hela cells
References
1 CofActor: A light- and stress-gated optogenetic clustering tool to study disease-associated cytoskeletal dynamics in living cells