Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00006
OS Name
Light-inducible transcriptional effectors (LITEs)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Transcription activator-like effectors
Detail Info
Recruited protein Virus protein 16
Detail Info
OS Application Information (1)
Mechanism of Action CRY2PHRCCIB1 heterodimerization [ 1]
Controlled Signal Pathway Endogenous transcription activation
Effect Activation
Application Optical control endogenous transcription and epigenetic states
Light Information Activation Wavelength 466 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Subseconds
Deactivation Time 15 min
Light Intensity 5 milliwatts per square centimeter
Expression Information Expression Method Transduction and Transfection
Cell Lines Mouse primary embryonic cortical neurons
References
1 Optical control of mammalian endogenous transcription and epigenetic states