Details of the Optogenetic Tool

General Information of This Photoreceptor (PR)
PR ID PR00010
PR Name Photolyase homology region of Arabidopsis Cryptochromes 2 (D387A) (CRY2PHR (D387A))
Source of Species Arabidopsis thalino
Class Cryptochromes
UniProt ID Q96524
GenBank NP_849588
PDB ID 6X24
Mutation Site D387A
Sequence
MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQFYPGRASRWWMKQSL
AHLSQSLKALGSDLTLIQTHNTISAILDCIRVTGPTKVVFNHLYDPVSLVRDHTVKEKLV
ERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSIESVMLPPPEEEGQFYPG
RWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLNEFIEKQLI
DYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGEESADLFLRG
IGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPLVDAGMRELWA
TGWMHNRIRVIVSSFGVKFLLLPWKWGMKYFWDTLLAADLECDILGWQYISGSIPDGHEL
DRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKASGVELGTNYAKP
IVDIDTARELLAKAISRTREAQIMIGAA
Components Photoreceptor Photolyase homology region of Arabidopsis Cryptochromes 2 (D387A) (CRY2PHR (D387A))
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Detail Info
Cofactor Flavin adenine dinucleotide (FAD)
Detail Info
3D Structure
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Complementary Protein Detail of This PR
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
UniProt ID A0A061DZ54
Sequence
MNRALPEMLQCSDMTVLERQRARLKWQQEQLQQQQLQQQEQQQSYFSELSGVFSSQPSHV
EGFQGGLMSGDSVLGDMVMTRQLKPDPGLETAWPELVKVDMPGMGFGPCGYGNGPSFDMN
YAISRTSSCPPAVAAAVAGEVVEVKGKESVVSEKIGSAVGRESFKKRKVDKLQNLKVVAE
DDSKRIKACAEEGESKITGPNTNKSSSNNNNNKKESSTDTSKENSKVSEVQKPDYIHVRA
RRGQATDSHSLAERVRREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQRQVEF
LSMKLAAVNPRLDFNVENLFAKEVFPSCTTNFPTVGMSSEMANPPYLQVSPVQHVVSCCG
LEMGMNTPDMAPRRTISAPVSIPDASFLDSSCFPQIQPSATWDVELQNLYNVAFDQGRST
SFPSQPFTGSIEASNLKMEM
Cofactor Detail of This PR
Cofactor Name Flavin adenine dinucleotide (FAD)
PubChem CID 643975
Structure
Formula C27H33N9O15P2
Molecular Weight 785.5
Optogenetic System (OS) Consisting of This PR
OS00073
OS Info
OS Name: Enhenced optogentic regulate stromal interaction molecule 1 system 1 (EOptoSTIM1-1)
[1]
Fusion protein: Stromal interaction molecule 1
Detail Info
OS00072
OS Info
OS Name: Optogenetic Controlled G-protein signaling system (Dark state) (Opto-RGS2(D387A))
[2]
Fusion protein: Human G-protein signaling 2
Detail Info
Recruited protein: Regulator of G-protein signaling 2
Detail Info
References
1 Optogenetic manipulation of calcium signals in single T cells in vivo
2 Optogenetic Inhibition of G(q) Protein Signaling Reduces Calcium Oscillation Stochasticity