Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00668
|
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| OS Name |
P21-CRY2/CIB1 system 5
(Opto-CRY2/CIB1 5)
|
[1] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Fusion protein | CDK-interacting protein p21 (CDKN1A)-nuclear localization signal (Arg 140, Lys 141 and Arg 142 replaced by Ala) | ||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2low-CIBN heteromerization | [ 1] | ||||
| Effect | Sensor | |||||
| Application | Make cells arrested in the G1 phase | |||||
| Note | CRY2low promote CRY2/CIBN heteromerization | |||||
| Light Information | Activation Wavelength | 450 nm | ||||
| Process | Reversible | |||||
| Activation Time | 0.5-2 min | |||||
| Light Intensity | 1-2 watt per square meter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | (CHO)-K1 cells, HEK293T | |||||