Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00627
|
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| OS Name |
Optogenetic Modulate intraocular pressure system
(OptoMIP)
|
[1] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Recruited protein | CAAX prenyl protease? | ||||
| Fusion protein | Inositol-polyphosphate-5-phosphatase OCRL | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2-CIBN heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Regulation PI(4,5)P2 levels | |||||
| Effect | Sensor | |||||
| Application | Optogenetic modulation of intraocular pressure in a glucocorticoid-induced ocular hypertension | |||||
| Note | Targeted modulation of OCRL may be used to decrease steroid-induced elevated elevated intraocular pressure | |||||
| Light Information | Activation Wavelength | 450 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | Minutes | |||||
| Deactivation Time | Minutes | |||||
| Light Intensity | 10 miliwatts | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | HTM cells | |||||