Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID	OS00538
OS Name	Optogenetic intracellular transport system 2 (Opto-IT 2)		[1]
Photoreceptor (PR) PR Info	PR Name Residue1-650 of Phytochrome B (PhyB (1-650)) Source of Species Arabidopsis Class Phytochromes UniProt ID P14713 GenBank NP_179469 PDB ID 4OUR 3D Structure Components Photoreceptor: Residue1-650 of Phytochrome B (PhyB (1-650)) Complementary Protein: Phytochrome interacting factor 6 (PIF6) Cofactor: Phycocyanobilin (PCB)
Complementary Protein	Phytochrome interacting factor 6 (PIF6)
Cofactor	Phycocyanobilin (PCB)
Controlled Protein	Recruited protein	Kinesin-1 heavy chain	Detail Info
Controlled Protein	Fusion protein	Ras-related protein Rab-11A	Detail Info

OS Application Information (1)
Mechanism of Action	PhyB-PIF6 heterodimerization		[ 1]
Controlled Signal Pathway	Target cargo transportation
Effect	Sensor
Application	Intracellular protein transport
Note	Dependence on PCB as a cofactor could be considered a limiting factor for this system
Light Information	Activation Wavelength	625 nm
	Process	Reversible
	Deactivation Wavelength	740 nm
	Activation Time	500 ms
	Deactivation Time	3.8 s
	Light Intensity	20 microwatts per square centimeter(625nm); 400 microwatts per square centimeter(740nm)
Expression Information	Expression Method	Transfection
Expression Information	Cell Lines	COS-7 and U2OS cells

References

A Phytochrome-Derived Photoswitch for Intracellular Transport