Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00227
OS Name
High-throughput screening of LOV protein system 20 (HTC-LOV 20)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (V416L) (AsLOV2 (V416L))
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
Mutation Site
V416L
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (V416L) (AsLOV2 (V416L))
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Functional protein LOV2 domain of Avena sativa Phototropin 1
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway Release the effector and confer it to active state
Effect Activation
Application High-throughput screening of fast- and slow- cycling LOV proteins
Note Slow cycling
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Thermal
Activation Time 10 s
Deactivation Time 160 s
Light Intensity 10 milliwatts per square centimeter
Expression Information Expression Method Transformation
Cell Lines E.coli
References
1 Fluorescence imaging-based high-throughput screening of fast- and slow-cycling LOV proteins