Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00070
OS Name
Enhenced optogentic regulate stromal interaction molecule 1 system 2 (EOptoSTIM1-2)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Cryptochromes 2 (E490G) (CRY2PHR (E490G))
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6K8I
Mutation Site
E490G
3D Structure
Components
Photoreceptor: Photolyase homology region of Cryptochromes 2 (E490G) (CRY2PHR (E490G))
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Stromal interaction molecule 1
Detail Info
OS Application Information (1)
Mechanism of Action CRY2PHR homo-oligomeirization [ 1]
Controlled Signal Pathway STIM1-gated Ca2+ influxion
Effect Activation
Application Manipulate calcium signals in single T cells in vivo
Note Calcium levels return to baseline levels but many T cells remain arrested
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Minutes
Deactivation Time Minutes
Light Intensity 710 milliwatts
Expression Information Expression Method Transduction
Cell Lines B3Z T cells and CD8+ T cells
References
1 Optogenetic manipulation of calcium signals in single T cells in vivo