Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00068
OS Name
Second-generation photoactivatable Cre recombinase (PA-Cre2.0)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Cryptochromes 2 (L348F) (CRY2PHR (L348F))
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
Mutation Site
L348F
3D Structure
Components
Photoreceptor: Photolyase homology region of Cryptochromes 2 (L348F) (CRY2PHR (L348F))
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein CreN means N terminal of Cre recombinase and CreC means C terminal of Cre recombinase
Detail Info
Recruited protein CreN means N terminal of Cre recombinase and CreC means C terminal of Cre recombinase
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIB1 heterodimerization [ 1]
Controlled Signal Pathway Transcription activation
Effect Activation
Application Five fold improved dynamic range than PA-Cre to control gene expression
Note 5-fold enhanced dynamic range and 50% reduced background levels than the original PA-Cre1.0
Light Information Activation Wavelength 461 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 1 s
Deactivation Time Minutes
Light Intensity 5.8 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines Yeast
References
1 Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase