Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00065
|
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| OS Name |
Optogenetic PKA system 4
(OptoPKA 4)
|
[1] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Fusion protein | A cAMP dependent protein kinase holoenzyme | ||||
| Recruited protein | Mitochondrial import receptor subunit TOM20 | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2-CIB1 heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Protein translocation | |||||
| Effect | Activation | |||||
| Application | Optogenetic control kinase activity | |||||
| Note | Reduced 5.6% activity than wild type | |||||
| Light Information | Activation Wavelength | 470 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | Seconds | |||||
| Deactivation Time | Minutes | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | MVD7 cells | |||||