Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00061
OS Name
Optogenetic BAX system (OptoBax)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Cryptochrome 2 (CRY2)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6K8I
3D Structure
Components
Photoreceptor: Cryptochrome 2 (CRY2)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein BCL2-associated X apoptosis regulator
Detail Info
Recruited protein Cryptochrome-interacting basic-helix-loop-helix 1
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIB1 heterodimerization [ 1]
Controlled Signal Pathway Phosphorylation of Bax
Effect Activation
Application Optogenetic contol cell apoptosis
Light Information Activation Wavelength 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Minutes
Expression Information Expression Method Transfection
Cell Lines Hela cells
References
1 Design, construction, and validation of optogenetic proteins