Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00058
|
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| OS Name |
Blue light control cell-cell interaction system
(BLCC)
|
[1] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Recruited protein | Cryptochrome-interacting basic-helix-loop-helix 1 | ||||
| Fusion protein | Platelet-derived growth factor receptor | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2-CIBN heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Selectively activate cell signal | |||||
| Effect | Activation | |||||
| Application | Using blue light to orthogonally control cell signal | |||||
| Note | PDGFR to anchor the protein to plasma membrane and CIBN was immobilized on functionalized glass substrates | |||||
| Light Information | Activation Wavelength | 480 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 30 min | |||||
| Deactivation Time | Within 2 hour | |||||
| Light Intensity | 80 microwatts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | MDA-MB-231 cells | |||||