Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00055
|
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| OS Name |
Brainbow-based live imaging system
(LOLlbow)
|
[1] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Fusion protein | CreN means N terminal of Cre recombinase and CreC means C terminal of Cre recombinase | ||||
| Recruited protein | CreN means N terminal of Cre recombinase and CreC means C terminal of Cre recombinase | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2PHRCCIB1 heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Cre mediated DNA recombinase | |||||
| Effect | Activation | |||||
| Application | Imaging muticolor-labeled cells in a non-invasive way | |||||
| Note | Permanent fluorescent labels for reveal morphological details | |||||
| Light Information | Activation Wavelength | Blue | ||||
| Process | Ireversible | |||||
| Activation Time | Minutes | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | Drosophila | |||||