Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00708
OS Name
LOV-Turbo precise spatiotemporal controling of proximity labeling (LOV-Turbo)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein TurboID (T52S; L73Q; I147T; A166V; I231V; F286L)
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Effect Sensor
Application Improve the temporal specificity of proximity labeling
Note LOV-Turbo can be activated by bioluminescence resonance energy transfer from luciferase
Light Information Activation Wavelength 470nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 15min
Deactivation Time Minutes
Light Intensity 0.11miliwatt per square centimeter
Expression Information Expression Method Transfection
Cell Lines HEK 293T cells
References
1 Engineered allostery in light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling in living cells