Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00700
OS Name
Photoactivatable targeted protein degradation system (paProtacL-Survivin)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Functional protein LOV2 domain of Avena sativa Phototropin 1
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway Survivin degradation
Effect Sensor
Application Survivin degradation
Note Survivin plays the role of anti-apoptosis and is exceedingly apparent in its expression in all tumour cells
Light Information Activation Wavelength Blue
Activation Time 48 h
Light Intensity 1.2 watts per square meter
Expression Information Expression Method Transfection
Cell Lines UMUC-3; 5637; T24; SW780; RT4 cells
References
1 Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells