Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00695
|
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| OS Name |
Optogenetic YAP
(optoYAP)
|
[1], [] | ||||
|
Photoreceptor (PR) |
||||||
| Cofactor | Flavin mononucleotide (FMN) | |||||
| Controlled Protein | Fusion protein | Yes-associated protein | ||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | J-alpha helix unfold | [ 1] , [ ] | ||||
| Controlled Signal Pathway | HippoYAP pathway | |||||
| Effect | Activation | |||||
| Application | Increase the speed of wound healing in cardiomyoblasts, driving cell migration. | |||||
| Note | Not increase in proliferation, but by collective cell migration | |||||
| Light Information | Activation Wavelength | 488 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 20 min | |||||
| Deactivation Time | 20 min | |||||
| Light Intensity | 0.15 miliwatts | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | H9c2; MKN28 cells | |||||