Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00687
OS Name
MPP8-LAMS, MPP8-based light-activated modification sensor (MPP8-LAMS)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein H3K9me3-binding reader domain of the human M phase phosphoprotein 8
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway Nuclear localization signal
Effect Sensor
Application Translocation of MPP8-LAMS into the nucleus
Light Information Activation Wavelength Blue
Process Reversible
Deactivation Wavelength Dark
Activation Time Minutes
Deactivation Time Minutes
Expression Information Expression Method Transfection
Cell Lines HeLa Kyoto cells
References
1 Optogenetics for sensors: On-demand fluorescent labeling of histone epigenetics