Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00669
OS Name
Light-Controlled p21 Applying the LINuS System (LINuS)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein CDK-interacting protein p21 (CDKN1A)-nuclear localization signal (Arg 140, Lys 141 and Arg 142 replaced by Ala)
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Effect Sensor
Application Make cells arrested in the G1 phase
Light Information Activation Wavelength 450 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Minutes
Light Intensity 1.33 watt per square meter
Expression Information Expression Method Transfection
Cell Lines (CHO)-K1 cells, HEK293T
References
1 Cell Cycle Control by Optogenetically Regulated Cell Cycle Inhibitor Protein p21