Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00644
OS Name
optoSynC, a tool foroptogeneticsynaptic vesicleclustering 1 (optoSynC 1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Cryptochromes 2 (1-535; E490G) (CRY2olig (535))
Source of Species
Arabidopsis thaliana
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
Mutation Site
E490G
3D Structure
Components
Photoreceptor: Cryptochromes 2 (1-535; E490G) (CRY2olig (535))
Cofactor: Flavin adenine dinucleotide (FAD)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Synaptic vesicles protein synaptogyrin
Detail Info
OS Application Information (1)
Mechanism of Action CRY2olig(535) homo-oligomerization [ 1]
Controlled Signal Pathway Movement of synaptic vesicles
Effect Inhibition
Application Inhibition of synaptic transmission
Note Does not affect ion channel currents, cellular biochemical processes, or synaptic proteins
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 5 s
Light Intensity 0.1-1 miliwatt per square milimeter
Expression Information Expression Method Microinjectin
Cell Lines Caenorhabditis elegans (C. elegans) neurons:pan-neuronal, cholinergic neurons, GABAergic neurons, PVD neurons; zebrafish neurons
References
1 Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles