Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00643
OS Name
LARIAT-based approach for synaptic vesicle clustering 2 (LARIAT 2)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Cryptochromes 2 (1-535; E490G) (CRY2olig (535))
Source of Species
Arabidopsis thaliana
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
Mutation Site
E490G
3D Structure
Components
Photoreceptor: Cryptochromes 2 (1-535; E490G) (CRY2olig (535))
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Functional protein Arabidopsis thalianaCryptochrome-2 variants with C-terminal charge alteration (535)
Detail Info
Recruited protein Synaptic vesicles protein synaptogyrin
Detail Info
OS Application Information (1)
Mechanism of Action CRY2olig(535)-CIBN heterodimerization [ 1]
Controlled Signal Pathway Movement of synaptic vesicles
Effect Inhibition
Application Inhibition of synaptic transmission
Note LARIAT-based approach did not affect swimming behavior in C. elegans
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Expression Information Expression Method Microinjectin
Cell Lines Caenorhabditis elegans (C. elegans) neurons:pan-neuronal, cholinergic neurons, GABAergic neurons, PVD neurons; zebrafish neurons
References
1 Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles