Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00637
OS Name
MyLOVChar (MyLOVChar)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Functional protein Myosin
Detail Info
OS Application Information (1)
Mechanism of Action J alpha helix unfold [ 1]
Controlled Signal Pathway Myosin motor
Effect Sensor
Application Fast and processive engineered myosins for protein motor controll
Note The movement continued with illumination time passed away
Light Information Activation Wavelength 470 nm
Process Reversible
Activation Time 150 s
Light Intensity 11 miliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines Mouse neurons; mouse embryonic fibroblast cell
References
1 Optical control of fast and processive engineered myosins in vitro and in living cells