Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00623
|
|||||
| OS Name |
Optogenetic control Exoc70 for membrane traffiking system 2
(Opto-Exoc70-MT 2)
|
[1] | ||||
|
Photoreceptor (PR) |
|
|||||
| Complementary Protein | Stringent starvation protein B (SspB) | |||||
| Cofactor | Flavin mononucleotide (FMN) | |||||
| Controlled Protein | Recruited protein | Exocyst complex component 70 | ||||
| Functional protein | Improved Light-Inducible Dimer | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | iLID-SspB heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Exocytosis | |||||
| Effect | Activation | |||||
| Application | Optotigenetic control Exo71 to study the role of the exocyst in tethering during exocytosis | |||||
| Note | iLID was tethered on membrane | |||||
| Light Information | Activation Wavelength | 488 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | Minutes | |||||
| Deactivation Time | Minutes | |||||
| Light Intensity | 1.5 watts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | Hela cells | |||||