Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00620
OS Name
Optogenetic regulate Voltage-gated potassium channel system (Opto-Kv1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Aureochromes 1 from Vaucheria frigida (AuLOV)
Source of Species
Vaucheria frigida
Class
LOV domain
UniProt ID
A8QW55
GenBank
BAF91488.1
PDB ID
3UE6
3D Structure
Components
Photoreceptor: Aureochromes 1 from Vaucheria frigida (AuLOV)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Potassium channel
Detail Info
OS Application Information (1)
Mechanism of Action AuLOV homodimerization [ 1]
Controlled Signal Pathway Potassium flow
Effect Activation
Application A novel photoactivatable Kv channels that provide new ways to interrogate neural circuits in vivo
Note AuLOV was fused to the N-terminus of the T1 domain
Current Intensity 9 uA
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 20 min
Deactivation Time 40 min
Light Intensity 14 microwatts per square milimeter
Expression Information Expression Method Transfection
Cell Lines HEK293T cells; CHO cells; CG cells; Xenopus oocytes
References
1 Light-regulated voltage-gated potassium channels for acute interrogation of channel function in neurons and behavior