Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00619
OS Name
Optogenetic regulate Toll-like Receptor 4 system (Opto-TLR4)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Aureochromes 1 from Vaucheria frigida (AuLOV)
Source of Species
Vaucheria frigida
Class
LOV domain
UniProt ID
A8QW55
GenBank
BAF91488.1
PDB ID
3UE6
3D Structure
Components
Photoreceptor: Aureochromes 1 from Vaucheria frigida (AuLOV)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Toll-like receptor 4
Detail Info
OS Application Information (1)
Mechanism of Action AuLOV homodimerization [ 1]
Controlled Signal Pathway TLR4 activation
Effect Activation
Application An ideal tool for investigating the underlying molecular mechanisms of TLR4 to find new thrapeutic options in pancreatic adenocarcinoma
Note TLR4 is closely associated with tumourigenesis and cancer progression
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 7.5 min
Deactivation Time 6 h
Light Intensity 7 volts
Expression Information Expression Method Transfection
Cell Lines Human pancreatic cancer cell line PANC-1
References
1 Light-Inducible Spatio-Temporal Control of TLR4 and NF-B-Gluc Reporter in Human Pancreatic Cell Line