Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00522
OS Name
Optogenetic control protein location system based on BphP1 (Opto-CPL-BphP1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Bacterial phytochrome photoreceptors 1 (Bphp1)
Source of Species
Rhodopseudomonas palustris
Class
Phytochromes
UniProt ID
A0A161I5N6
GenBank
1569047741
PDB ID
5OY5
3D Structure
Components
Photoreceptor: Bacterial phytochrome photoreceptors 1 (Bphp1)
Complementary Protein: Transcriptional regulator PpsR2 (PpsR2)
Cofactor: Biliverdin (BV)
Complementary Protein Transcriptional regulator PpsR2 (PpsR2)
Cofactor Biliverdin (BV)
Controlled Protein Fusion protein Intersectin (ITSN) guanine exchange factor (GEF) catalytic domain
Detail Info
Recruited protein Transcriptional regulator PpsR2
Detail Info
OS Application Information (1)
Mechanism of Action BphP1-PpsR2 heterodimerization [ 1]
Controlled Signal Pathway Protein translocation
Effect Activation
Application Subcellular protein targeting; induction of intracellular enzymatic activity and activation of gene expression
Note Activation and deactivation time varies with light intensity
Light Information Activation Wavelength 740 nm
Process Reversible
Deactivation Wavelength 636 nm or dark(slower)
Activation Time 28 s
Deactivation Time 21 s (636 nm) or 900 s (dark)
Light Intensity 1 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines Hela cells
OS Application Information (2)
Mechanism of Action BphP1-PpsR2 heterodimerization [ 1]
Controlled Signal Pathway Protein translocation
Effect Activation
Application Subcellular protein targeting; induction of intracellular enzymatic activity and activation of gene expression
Note Activation and deactivation time varies with light intensity
Light Information Activation Wavelength 740 nm
Process Reversible
Deactivation Wavelength 636 nm or dark (slower)
Activation Time 3.5 s
Deactivation Time 3.0 s (636 nm) or 900 s (dark)
Light Intensity 27 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines Hela cells
References
1 A bacterial phytochrome-based optogenetic system controllable with near-infrared light