Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID	OS00516
OS Name	Optogenetic control c-di-GMP synthetic system (Opto-c-di-GMP)		[1]
Photoreceptor (PR) PR Info	PR Name BphS (BphS) Source of Species Rhodobacter sphaeroides Class Phytochromes 3D Structure Components Photoreceptor: BphS (BphS) Cofactor: Phycocyanobilin (PCB)
Cofactor	Phycocyanobilin (PCB)
Controlled Protein	Recruited protein	Beta-galactosidase	Detail Info
	Fusion protein	Heme oxygenase	Detail Info
	Fusion protein	The bacterial c-di-GMP phosphodiesterase	Detail Info

OS Application Information (1)
Mechanism of Action	c-di-GMP synthesis/degradation		[ 1]
Controlled Signal Pathway	Gene expression activation
Effect	Activation
Application	Near-infrared light responsive synthetic c-di-GMP module for optogenetic application
Note	BphG-GGDEF domain (in slr1143)=BphS
Light Information	Activation Wavelength	712 nm
	Process	Reversible
	Deactivation Wavelength	756 nm
	Activation Time	3 h
	Light Intensity	0.2 milliwatts per square centimeter
Expression Information	Expression Method	Transfection
Expression Information	Cell Lines	E.coli

References

Near-infrared light responsive synthetic c-di-GMP module for optogenetic applications