Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00442
OS Name
Gloeobacter violaceus rhodopsin variants study system 1 (GR-VS 1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Gloeobacter violaceus rhodopsin (G162L) (GR (G162L))
Source of Species
Gloeobacter violaceus
Class
Opsin
UniProt ID
Q7NP59
GenBank
499452738
PDB ID
6NWD
Mutation Site
G162L
3D Structure
Components
Photoreceptor: Gloeobacter violaceus rhodopsin (G162L) (GR (G162L))
Cofactor: All-trans-Retinal (Trans-Retinal)
Cofactor All-trans-Retinal (Trans-Retinal)
Controlled Protein Functional protein Gloeobacter violaceus rhodopsin
Detail Info
OS Application Information (1)
Mechanism of Action Ion channel open [ 1]
Controlled Signal Pathway Fluorescence indicator
Effect Sensor
Application Proton-pumping rhodopsins can work as fluorescent sensor of membrane potential
Note Blue shifted GR abtained by mutation
Channel Type Proton pump
Light Information Activation Wavelength 496 nm
Expression Information Expression Method Transformation
Cell Lines E.coli
References
1 Directed evolution of Gloeobacter violaceus rhodopsin spectral properties