Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00346
|
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| OS Name |
Optogenetic regulate Galpha activity system 3
(Opto-Galpha 3)
|
[1] | ||||
|
Photoreceptor (PR) |
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| Complementary Protein | Positive Magnet (pMag) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Recruited protein | Guanine nucleotide-binding protein G(q) subunit alpha | ||||
| Functional protein | Negative Magnet | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | nMag-pMag heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Protein translocation | |||||
| Effect | Sensor | |||||
| Application | Blue light induced membrane recruitment of Galphaq using the magnet system | |||||
| Light Information | Activation Wavelength | 488 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 50 ms | |||||
| Light Intensity | 1 milliwatts | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | COS-7 cells | |||||