Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00205
OS Name
Optogenetic regulation of peroxisomal protein import system (Opto-PPI)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (G528A; N538E) (AsLOV2 (G528A; N538E))
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
Mutation Site
G528A; N538E
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (G528A; N538E) (AsLOV2 (G528A; N538E))
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Acyl-CoA oxidase sequence
Detail Info
Nuclear export sequence
Detail Info
Recruited protein Peroxisomal import receptor
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold and PTS1-Pex5 heterodimerization [ 1]
Controlled Signal Pathway Peroxisomal protein trafficking
Effect Sensor
Application Optical control of peroxisomal trafficking
Note Rapamycin-induced dimerization can block light-triggered peroxisomal import of GFP-LOV-PTS1
Light Information Activation Wavelength 461 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Minutes
Light Intensity 5.8 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines COS-7 cells; HEK293T cells and Hela cells
References
1 Optical Control of Peroxisomal Trafficking