Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00192
OS Name
Optogenetic control Rac system (Opto-Rac)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Improved Light-Inducible Dimer (iLID)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
747155673
PDB ID
4WF0
3D Structure
Components
Photoreceptor: Improved Light-Inducible Dimer (iLID)
Complementary Protein: Stringent starvation protein B (SspB)
Cofactor: Flavin mononucleotide (FMN)
Complementary Protein Stringent starvation protein B (SspB)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Recruited protein Endogenous Rac1 protein
Detail Info
Functional protein Improved Light-Inducible Dimer
Detail Info
OS Application Information (1)
Mechanism of Action ILID comformational change and subsequently iLID-SspB heterodimerization [ 1]
Controlled Signal Pathway Rac1 activation
Effect Sensor
Application Optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration
Note Not need exogenous fibronectin yet require integrin binding and myosin contractility
Light Information Activation Wavelength 467 nm
Activation Time Seconds
Expression Information Expression Method Transfection
Cell Lines Mouse IA32 fibroblasts
References
1 Cells lay their own tracks - optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration