Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00191
|
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| OS Name |
Optogenetic control RhoA system
(Opto-RhoA)
|
[1], [2] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Stringent starvation protein B (SspB) | |||||
| Cofactor | Flavin mononucleotide (FMN) | |||||
| Controlled Protein | Functional protein | Improved Light-Inducible Dimer | ||||
| Recruited protein | Rho guanine nucleotide exchange factor 12 | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | ILID comformational change and subsequently iLID-SspB heterodimerization | [ 1] , [ 2] | ||||
| Controlled Signal Pathway | RhoA activation | |||||
| Effect | Sensor | |||||
| Application | Optogenetic control of small GTPases reveals RhoA mediates intracellular calcium signaling | |||||
| Note | RhoA activate calcium increases varies between different cells | |||||
| Light Information | Activation Wavelength | 458 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | Seconds to Minutes | |||||
| Deactivation Time | Seconds | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | MDCK cells; HEK293T cells; RPE1 cells and HeLa cells | |||||