Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID	OS00190
OS Name	Light-induceble tethering system (LIT)		[1]
Photoreceptor (PR) PR Info	PR Name Improved Light-Inducible Dimer (iLID) Source of Species Avena sativa Class LOV domain UniProt ID O49003 GenBank 747155673 PDB ID 4WF0 3D Structure Components Photoreceptor: Improved Light-Inducible Dimer (iLID) Complementary Protein: Stringent starvation protein B (SspB) Cofactor: Flavin mononucleotide (FMN)
Complementary Protein	Stringent starvation protein B (SspB)
Cofactor	Flavin mononucleotide (FMN)
Controlled Protein	Recruited protein	Cytochrome b5	Detail Info
Controlled Protein	Fusion protein	OMM linker peptide sequence	Detail Info

OS Application Information (1)
Mechanism of Action	ILID comformational change and subsequently iLID-SspB heterodimerization		[ 1]
Controlled Signal Pathway	Protein translocation
Effect	Sensor
Application	Optogenetic control of endoplasmic reticulum-mitochondria tethering
Note	The first time examination of whether ER-mitochondria contacts can regulate well-known cellular events
Light Information	Activation Wavelength	488 nm
	Process	Reversible
	Deactivation Wavelength	Dark
	Activation Time	1 s
	Deactivation Time	90 s
Expression Information	Expression Method	Transfection
Expression Information	Cell Lines	NIH 3T3 and HEK 293T cells

References

Optogenetic Control of Endoplasmic Reticulum-Mitochondria Tethering